Molecular detection of Babesia bovis along with haemato-biochemical alterations in large ruminants associated with babesiosis in central Punjab, Pakistan

Babesiosis is endemic in Pakistan and one of the most important bovine diseases that cause huge economic losses high mortality young animals. A hematobiochemical study was conducted to unveil difference between diseased healthy animals specific districts i.e., Faisalabad, Toba Tek Singh Jhang Punjab, Pakistan. The total number blood samples collected were 518 which include 158 buffaloes 360 cattle. are then analyzed with help PCR (Polymerase Chain Reaction) targeting apocytochrome b-genes (CYTb) followed by analysis. analysis data done Chi-square test. tests summer suggests 53 out 180 (29.4%) cows 19 79 (24.05%) prone babesiosis. On other hand, winter results showed 12.7 (23/180), 13.92 % (11/79) positive for Babesia genus through c-PCR. further investigated hematological biochemical revealed that, mean value parameters like RBCs, Hb, PCV, MCV MCHC significantly (P< 0.05) decreased infected (cows buffaloes) as compared non-infected ones. While ALT, AST, Cholesterol LDH increased These findings novel molecular evidence B. bovis dairy herds Punjab province,